Hotspot

accD-psaI

LSC · 63235-64226 · 992 bp

Back to Colocasia

Pi 0.00555

MarkerSeek score 63.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 992
variable_sites: 11
pi: 0.00555
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCACAAGCGGCTGAGTATTC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1182-1209 bp	1.0	81.0
accD-psaI_p2	GCGGCTGAGTATTCATTCCA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1176-1203 bp	1.0	80.8
accD-psaI_p3	TCACAAGCGGCTGAGTATTC	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	1188-1215 bp	1.0	80.4
accD-psaI_p4	GCGGCTGAGTATTCATTCCA	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	1182-1209 bp	1.0	80.1
accD-psaI_p5	AGCGGCTGAGTATTCATTCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	1177-1204 bp	1.0	79.2