Hotspot

rbcL-accD

LSC · 60987-61758 · 772 bp

Back to Colocasia

Pi 0.00195

MarkerSeek score 60.40

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 772
variable_sites: 3
pi: 0.00195
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CCGTGAAGCTAGCAAATGGA	GTCTTCGCTTCCACTGCTAT	50.0	50.0	58.3	57.7	997-1002 bp	1.0	75.9
rbcL-accD_p2	TCCGTGAAGCTAGCAAATGG	GTCTTCGCTTCCACTGCTAT	50.0	50.0	58.3	57.7	998-1003 bp	1.0	75.9
rbcL-accD_p3	GAGTCCTGAACTAGCTGCTG	GTCTTCGCTTCCACTGCTAT	55.0	50.0	57.7	57.7	979-984 bp	1.0	75.7
rbcL-accD_p4	GCTAGCAAATGGAGTCCTGA	GTCTTCGCTTCCACTGCTAT	50.0	50.0	57.3	57.7	990-995 bp	1.0	72.4
rbcL-accD_p5	AGCTAGCAAATGGAGTCCTG	GTCTTCGCTTCCACTGCTAT	50.0	50.0	57.3	57.7	991-996 bp	1.0	72.3