Hotspot

tRNA-Thr(UGU)-tRNA-Leu(UAA)

LSC · 49778-50919 · 1142 bp

Back to Colocasia

Pi 0.00306

MarkerSeek score 62.10

Primer available yes

Label tRNA-Thr(UGU)-tRNA-Leu(UAA)

Feature Metadata

label_name: tRNA-Thr(UGU)-tRNA-Leu(UAA)
feature_type: igs
region: LSC
length_bp: 1142
variable_sites: 7
pi: 0.00306
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Thr(UGU)-tRNA-Leu(UAA)_p1	GGAAAAAGCCAGCTATCGGA	TCCATACCAAGGCTCAATGC	50.0	50.0	58.0	57.9	1280-1311 bp	1.0	80.4
tRNA-Thr(UGU)-tRNA-Leu(UAA)_p2	AGGGAAAAAGCCAGCTATCG	TCCATACCAAGGCTCAATGC	50.0	50.0	58.0	57.9	1282-1313 bp	1.0	80.4
tRNA-Thr(UGU)-tRNA-Leu(UAA)_p3	GGAAAAAGCCAGCTATCGGA	ACCAAGGCTCAATGCAATCA	50.0	45.0	58.0	58.1	1275-1306 bp	1.0	80.3
tRNA-Thr(UGU)-tRNA-Leu(UAA)_p4	AGGGAAAAAGCCAGCTATCG	ACCAAGGCTCAATGCAATCA	50.0	45.0	58.0	58.1	1277-1308 bp	1.0	80.2
tRNA-Thr(UGU)-tRNA-Leu(UAA)_p5	GGAAAAAGCCAGCTATCGGA	GCGTCTACCTATTTCGCCAT	50.0	50.0	58.0	57.8	1247-1278 bp	1.0	79.7