Hotspot

tRNA-Tyr(GUA)-tRNA-Glu(UUC)

LSC · 33646-33805 · 160 bp

Back to Colocasia

Pi 0.0167

MarkerSeek score 66.10

Primer available yes

Label tRNA-Tyr(GUA)-tRNA-Glu(UUC)

Feature Metadata

label_name: tRNA-Tyr(GUA)-tRNA-Glu(UUC)
feature_type: igs
region: LSC
length_bp: 160
variable_sites: 5
pi: 0.0167
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Tyr(GUA)-tRNA-Glu(UUC)_p1	AGAAAGCGGCGAATTTTTGG	TAGCGGTTCAGGACATCTCT	45.0	50.0	57.9	57.9	322-339 bp	1.0	80.2
tRNA-Tyr(GUA)-tRNA-Glu(UUC)_p2	AGAAAGCGGCGAATTTTTGG	TCTAGCGGTTCAGGACATCT	45.0	50.0	57.9	57.9	324-341 bp	1.0	80.2
tRNA-Tyr(GUA)-tRNA-Glu(UUC)_p3	AGAAAGCGGCGAATTTTTGG	GTCTAGCGGTTCAGGACATC	45.0	55.0	57.9	57.8	325-342 bp	1.0	79.7
tRNA-Tyr(GUA)-tRNA-Glu(UUC)_p4	TACTCAAAAGAAAGCGGCGA	TAGCGGTTCAGGACATCTCT	45.0	50.0	57.8	57.9	330-347 bp	1.0	79.4
tRNA-Tyr(GUA)-tRNA-Glu(UUC)_p5	TACTCAAAAGAAAGCGGCGA	TCTAGCGGTTCAGGACATCT	45.0	50.0	57.8	57.9	332-349 bp	1.0	79.4