Hotspot

petN-psbM

LSC · 31385-32609 · 1225 bp

Back to Colocasia

Pi 0.00368

MarkerSeek score 61.40

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1225
variable_sites: 9
pi: 0.00368
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTATGGGGAAGGAGTGGAC	TGCTACTGCATTGTTTATTCT	55.0	33.3	58.1	53.5	1328-1329 bp	1.0	40.6
petN-psbM_p2	GTATGGGGAAGGAGTGGACT	TGCTACTGCATTGTTTATTCT	55.0	33.3	58.1	53.5	1327-1328 bp	1.0	40.6
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCATTGTTTATTCT	50.0	33.3	58.2	53.5	1386-1387 bp	1.0	40.6
petN-psbM_p4	AGTATGGGGAAGGAGTGGAC	TGCTACTGCATTGTTTATTCTAGT	55.0	33.3	58.1	56.4	1328-1329 bp	1.0	40.6
petN-psbM_p5	GTATGGGGAAGGAGTGGACT	TGCTACTGCATTGTTTATTCTAGT	55.0	33.3	58.1	56.4	1327-1328 bp	1.0	40.6