Hotspot

rpoB-tRNA-Cys(GCA)

LSC · 28919-30349 · 1431 bp

Back to Colocasia

Pi 0.00519

MarkerSeek score 62.70

Primer available yes

Label rpoB-tRNA-Cys(GCA)

Feature Metadata

label_name: rpoB-tRNA-Cys(GCA)
feature_type: igs
region: LSC
length_bp: 1431
variable_sites: 14
pi: 0.00519
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-tRNA-Cys(GCA)_p1	CCCTCATTTTCATCCCGGAG	GCGACACCCAGATTTGAACT	55.0	50.0	58.0	58.5	1523-1568 bp	1.0	79.3
rpoB-tRNA-Cys(GCA)_p2	CTCATTTTCATCCCGGAGCA	GCGACACCCAGATTTGAACT	50.0	50.0	58.0	58.5	1521-1566 bp	1.0	79.2
rpoB-tRNA-Cys(GCA)_p3	CCCTCATTTTCATCCCGGAG	TAAAGGATTTGCAGTCCCCC	55.0	50.0	58.0	57.5	1498-1543 bp	1.0	79.0
rpoB-tRNA-Cys(GCA)_p4	CCCTCATTTTCATCCCGGAG	CCTTACCACTTGGCCATGTC	55.0	55.0	58.0	58.5	1477-1522 bp	1.0	78.9
rpoB-tRNA-Cys(GCA)_p5	CTCATTTTCATCCCGGAGCA	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.0	57.5	1496-1541 bp	1.0	78.8