Hotspot

psbA-tRNA-Lys(UUU)

LSC · 1754-2014 · 261 bp

Back to Colocasia

Pi 0.00447

MarkerSeek score 60.70

Primer available yes

Label psbA-tRNA-Lys(UUU)

Feature Metadata

label_name: psbA-tRNA-Lys(UUU)
feature_type: igs
region: LSC
length_bp: 261
variable_sites: 2
pi: 0.00447
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-tRNA-Lys(UUU)_p1	TAGGCTTGTACTTTCGCGTC	TCGTGTGCAATGAAAACTGC	50.0	45.0	58.0	57.9	406-424 bp	1.0	83.3
psbA-tRNA-Lys(UUU)_p2	TAGGCTTGTACTTTCGCGTC	AATGAAAACTGCAAGCACGG	50.0	45.0	58.0	57.9	398-416 bp	1.0	83.2
psbA-tRNA-Lys(UUU)_p3	TAGGCTTGTACTTTCGCGTC	CGTGTGCAATGAAAACTGCA	50.0	45.0	58.0	58.2	405-423 bp	1.0	83.0
psbA-tRNA-Lys(UUU)_p4	ATGTAAAGGCGGTTTTCGGT	TCGTGTGCAATGAAAACTGC	45.0	45.0	58.1	57.9	456-474 bp	1.0	82.2
psbA-tRNA-Lys(UUU)_p5	ATGTAAAGGCGGTTTTCGGT	AATGAAAACTGCAAGCACGG	45.0	45.0	58.1	57.9	448-466 bp	1.0	82.1