Hotspot

trnH-psbA

LSC · 69-691 · 623 bp

Back to Colocasia

Pi 0.0154

MarkerSeek score 67.50

Primer available yes

Label trnH-psbA

Feature Metadata

label_name: trnH-psbA
feature_type: igs
region: LSC
length_bp: 623
variable_sites: 17
pi: 0.0154
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-psbA_p1	GATCCACTTGGCTACATCCG	CAACCGTGCTAACCTTGGTA	55.0	50.0	58.1	57.8	537-745 bp	1.0	83.6
trnH-psbA_p2	CAATCCACTGCCTTGATCCA	CAACCGTGCTAACCTTGGTA	50.0	50.0	57.9	57.8	551-759 bp	1.0	83.5
trnH-psbA_p3	AATCCACTGCCTTGATCCAC	CAACCGTGCTAACCTTGGTA	50.0	50.0	57.9	57.8	550-758 bp	1.0	83.5
trnH-psbA_p4	ACAATCCACTGCCTTGATCC	CAACCGTGCTAACCTTGGTA	50.0	50.0	57.9	57.8	552-760 bp	1.0	83.5
trnH-psbA_p5	GATCCACTTGGCTACATCCG	ATCAACCGTGCTAACCTTGG	55.0	50.0	58.1	58.2	539-747 bp	1.0	83.5