Hotspot

ycf1

SSC · 29939-35476 · 5538 bp

Back to Colobanthus

Pi 0.00433

MarkerSeek score 36.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5538
variable_sites: 71
pi: 0.00433
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCATGTGATTAGGGATTGTCGA	AGTTTACAAAGAAATGGTTTCGA	45.5	30.4	57.9	55.0	225 bp	1.0	43.2
ycf1_p2	ACCATGTGATTAGGGATTGTCG	AGTTTACAAAGAAATGGTTTCGA	45.5	30.4	58.2	55.0	226 bp	1.0	43.2
ycf1_p3	CCATGTGATTAGGGATTGTCGA	AAGTTTACAAAGAAATGGTTTCGA	45.5	29.2	57.9	55.7	226 bp	1.0	43.2
ycf1_p4	ACCATGTGATTAGGGATTGTCG	AAGTTTACAAAGAAATGGTTTCGA	45.5	29.2	58.2	55.7	227 bp	1.0	43.2
ycf1_p5	CCATGTGATTAGGGATTGTCG	AGTTTACAAAGAAATGGTTTCGA	47.6	30.4	56.5	55.0	225 bp	1.0	43.2