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psbT-psbN

LSC · 132550-132620 · 71 bp

Back to Colobanthus

Pi 0.0602

MarkerSeek score 84.90

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 71
variable_sites: 8
pi: 0.0602
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	AACCTCCGAAAGTTCCAACT	ATCCTTTCGAAGAACACGGG	45.0	50.0	57.0	57.9	126 bp	0.857143	70.4
psbT-psbN_p2	GAACCTCCGAAAGTTCCAACT	ATCCTTTCGAAGAACACGGG	47.6	50.0	58.2	57.9	127 bp	0.857143	69.3
psbT-psbN_p3	AACCTCCGAAAGTTCCAACT	TTCGAAGAACACGGGGACTA	45.0	50.0	57.0	58.4	121 bp	0.857143	68.3
psbT-psbN_p4	GAACCTCCGAAAGTTCCAAC	ATCCTTTCGAAGAACACGGG	50.0	50.0	56.7	57.9	127 bp	0.857143	67.8
psbT-psbN_p5	GAACCTCCGAAAGTTCCAACT	TTCGAAGAACACGGGGACTA	47.6	50.0	58.2	58.4	122 bp	0.857143	67.3