Hotspot

rpl33-rps18

LSC · 126019-126299 · 281 bp

Back to Colobanthus

Pi 0.013

MarkerSeek score 66.60

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 281
variable_sites: 9
pi: 0.013
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	GGCACAATACACCTAGTCGG	TAATCAATTCGATCCCCCGG	55.0	50.0	58.1	57.1	428-465 bp	1.0	78.3
rpl33-rps18_p2	GGCACAATACACCTAGTCGG	GCAAACGCCTACGAACAGAT	55.0	50.0	58.1	58.9	397-434 bp	1.0	77.8
rpl33-rps18_p3	GGCACAATACACCTAGTCGG	CAAACGCCTACGAACAGATC	55.0	50.0	58.1	56.9	396-433 bp	1.0	76.7
rpl33-rps18_p4	GGCACAATACACCTAGTCGG	AATCAATTCGATCCCCCGGT	55.0	50.0	58.1	59.2	427-464 bp	1.0	75.9
rpl33-rps18_p5	GGCACAATACACCTAGTCGG	ATCAATTCGATCCCCCGGTT	55.0	50.0	58.1	59.2	426-463 bp	1.0	75.9