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psbE-petL

LSC · 122789-124046 · 1258 bp

Back to Colobanthus

Pi 0.00653

MarkerSeek score 61.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1258
variable_sites: 27
pi: 0.00653
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCAGTGCTTC	AAGCTGTAGTTAAAGCCGCT	50.0	45.0	57.8	57.5	1328-1340 bp	1.0	78.1
psbE-petL_p2	ACGAATAACCAACCCGCAAT	AAGCTGTAGTTAAAGCCGCT	45.0	45.0	58.2	57.5	1414-1426 bp	1.0	77.8
psbE-petL_p3	TGCTGACGAATAACCAACCC	AAGCTGTAGTTAAAGCCGCT	50.0	45.0	58.2	57.5	1419-1431 bp	1.0	77.7
psbE-petL_p4	GTTCTCCAGTGCTTCCAGAC	AAGCTGTAGTTAAAGCCGCT	55.0	45.0	58.2	57.5	1323-1335 bp	1.0	77.7
psbE-petL_p5	CGAATAACCAACCCGCAATG	AAGCTGTAGTTAAAGCCGCT	50.0	45.0	57.8	57.5	1413-1425 bp	1.0	77.6