Hotspot

rps4-trnT-UGU

LSC · 103663-104076 · 414 bp

Back to Colobanthus

Pi 0.00611

MarkerSeek score 55.10

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 414
variable_sites: 8
pi: 0.00611
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CTCCTAGGCGGCGTATTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	515-526 bp	1.0	81.5
rps4-trnT-UGU_p2	CTCCTAGGCGGCGTATTTTT	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.1	57.8	483-494 bp	1.0	80.8
rps4-trnT-UGU_p3	GCTCTAAGATCACTTCCGGC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.1	57.9	567-578 bp	1.0	80.5
rps4-trnT-UGU_p4	CTCCTAGGCGGCGTATTTTT	TAGAATTTCTAGCAGCCCGC	50.0	50.0	58.1	57.8	539-550 bp	1.0	80.5
rps4-trnT-UGU_p5	GCGTGATTGGGCTCTAAGAT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.8	57.9	577-588 bp	1.0	79.6