Hotspot

psaA-ycf3

LSC · 99566-100673 · 1108 bp

Back to Colobanthus

Pi 0.00684

MarkerSeek score 64.80

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 1108
variable_sites: 22
pi: 0.00684
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GTTCTTGAAAAATGGCCGGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	1296-1347 bp	1.0	83.6
psaA-ycf3_p2	GTTCTTGAAAAATGGCCGGG	TTGGTTGAAGATCACGGGAC	50.0	50.0	57.9	57.8	1229-1280 bp	1.0	82.7
psaA-ycf3_p3	TTCTTGAAAAATGGCCGGGT	ATCAAGCCGCTGAGTATTGG	45.0	50.0	58.3	58.0	1295-1346 bp	1.0	81.9
psaA-ycf3_p4	GTTCTTGAAAAATGGCCGGG	AGCCGCTGAGTATTGGAAAC	50.0	50.0	57.9	58.3	1292-1343 bp	1.0	81.9
psaA-ycf3_p5	GTTCTTGAAAAATGGCCGGG	CAAGCCGCTGAGTATTGGAA	50.0	50.0	57.9	58.3	1294-1345 bp	1.0	81.8