Hotspot

petN-psbM

LSC · 86771-87356 · 586 bp

Back to Colobanthus

Pi 0.0111

MarkerSeek score 52.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 586
variable_sites: 15
pi: 0.0111
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATTGTAAGTCTCGCTTGGGC	AAAACGGTCAGCCAAAATGA	50.0	40.0	58.3	56.4	685-694 bp	1.0	69.2
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	AAAACGGTCAGCCAAAATGA	50.0	40.0	57.6	56.4	623-632 bp	1.0	68.8
petN-psbM_p3	ATTGTAAGTCTCGCTTGGGC	AAACGGTCAGCCAAAATGAT	50.0	40.0	58.3	56.2	684-693 bp	1.0	67.3
petN-psbM_p4	ATTGTAAGTCTCGCTTGGGC	AACGGTCAGCCAAAATGATT	50.0	40.0	58.3	56.2	683-692 bp	1.0	67.3
petN-psbM_p5	ATTGTAAGTCTCGCTTGGGC	AAAAACGGTCAGCCAAAATGA	50.0	38.1	58.3	57.2	686-695 bp	1.0	67.1