Hotspot

trnS-GCU-trnG-UCC

LSC · 66875-67638 · 764 bp

Back to Colobanthus

Pi 0.00675

MarkerSeek score 56.70

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 764
variable_sites: 16
pi: 0.00675
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CACGGAAAGAGAGGGATTCG	CCGAGAGACCCCCTAACTAT	55.0	55.0	58.1	57.3	922-928 bp	1.0	75.7
trnS-GCU-trnG-UCC_p2	CACGGAAAGAGAGGGATTCG	ACCGAGAGACCCCCTAACTA	55.0	55.0	58.1	58.7	923-929 bp	1.0	75.6
trnS-GCU-trnG-UCC_p3	CACGGAAAGAGAGGGATTCG	AAACCGAGAGACCCCCTAAC	55.0	55.0	58.1	58.7	925-931 bp	1.0	75.3
trnS-GCU-trnG-UCC_p4	CACGGAAAGAGAGGGATTCG	CAAACCGAGAGACCCCCTAA	55.0	55.0	58.1	58.7	926-932 bp	1.0	75.3
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	CCGAGAGACCCCCTAACTAT	50.0	55.0	58.2	57.3	921-927 bp	1.0	75.1