Hotspot

trnK-UUU-rps16

LSC · 63209-63763 · 555 bp

Back to Colobanthus

Pi 0.0123
MarkerSeek score 55.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
555
variable_sites
18
pi
0.0123
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGAATCCCTTGCTTCATCCA CGCTCAACCAACAGAAACTG 50.0 50.0 58.0 57.6 725-866 bp 1.0 78.6
trnK-UUU-rps16_p2 ACGAATCCCTTGCTTCATCC CGCTCAACCAACAGAAACTG 50.0 50.0 58.0 57.6 726-867 bp 1.0 78.6
trnK-UUU-rps16_p3 CGAATCCCTTGCTTCATCCA ATTCAAAAAGGCGCTCAACC 50.0 45.0 58.0 57.6 736-877 bp 1.0 78.2
trnK-UUU-rps16_p4 ACGAATCCCTTGCTTCATCC ATTCAAAAAGGCGCTCAACC 50.0 45.0 58.0 57.6 737-878 bp 1.0 78.2
trnK-UUU-rps16_p5 CGATGGTGTAGACGAATCCC CGCTCAACCAACAGAAACTG 55.0 50.0 57.9 57.6 737-878 bp 1.0 77.8