Hotspot

trnH-GUG-psbA

LSC · 59046-59308 · 263 bp

Back to Colobanthus

Pi 0.0246

MarkerSeek score 72.10

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 263
variable_sites: 17
pi: 0.0246
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	309-334 bp	1.0	86.5
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCGAT	55.0	55.0	58.1	58.1	306-331 bp	1.0	86.4
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	323-348 bp	1.0	86.3
trnH-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	322-347 bp	1.0	86.3
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	324-349 bp	1.0	86.3