Hotspot

ndhF-rpl32

SSC · 18630-19510 · 881 bp

Back to Colobanthus

Pi 0.0067

MarkerSeek score 40.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 881
variable_sites: 16
pi: 0.0067
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCGTATTGGTATATATGTTCCA	AGACTTTTTGGAAATAGAAGTACGT	36.4	32.0	53.6	56.7	945-950 bp	1.0	41.4
ndhF-rpl32_p2	GCGTATTGGTATATATGTTCCA	GACTTTTTGGAAATAGAAGTACGT	36.4	33.3	53.6	55.4	944-949 bp	1.0	41.4
ndhF-rpl32_p3	GCGTATTGGTATATATGTTCCA	AGACTTTTTGGAAATAGAAGTACGTT	36.4	30.8	53.6	57.3	945-950 bp	1.0	41.4
ndhF-rpl32_p4	GCGTATTGGTATATATGTTCCAT	AGACTTTTTGGAAATAGAAGTACGT	34.8	32.0	54.2	56.7	945-950 bp	1.0	41.4
ndhF-rpl32_p5	GCGTATTGGTATATATGTTCCA	ACTTTTTGGAAATAGAAGTACGT	36.4	30.4	53.6	54.2	943-948 bp	1.0	41.4