Hotspot

psbC

LSC · 33525-34946 · 1422 bp

Back to Collinsonia

Pi 0.00188

MarkerSeek score 59.90

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 4
pi: 0.00188
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	AGGTATTCGTGCTTGGATGG	AGTGATTCCTACTTCAATTCAAGGA	50.0	36.0	57.7	58.0	1504 bp	1.0	40.2
psbC_p2	AAATTCGTGCAGCGGAAGAT	AGTGATTCCTACTTCAATTCAAGGA	45.0	36.0	58.5	58.0	1568 bp	1.0	40.2
psbC_p3	TCTCAGGAAATTCGTGCAGC	AGTGATTCCTACTTCAATTCAAGGA	50.0	36.0	58.6	58.0	1575 bp	1.0	40.2
psbC_p4	AGGTATTCGTGCTTGGATGG	GTGATTCCTACTTCAATTCAAGGA	50.0	37.5	57.7	56.7	1503 bp	1.0	40.2
psbC_p5	AGGTATTCGTGCTTGGATGG	AGTGATTCCTACTTCAATTCAAGG	50.0	37.5	57.7	56.7	1504 bp	1.0	40.2