Hotspot

petG-trnW-CCA

LSC · 66240-66366 · 127 bp

Back to Collinsonia

Pi 0.021

MarkerSeek score 60.40

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 127
variable_sites: 4
pi: 0.021
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	GCGATCAGTTGGACCTTTGA	AGAACGTGGGTCTCCAAAAC	50.0	50.0	58.2	58.0	199-200 bp	1.0	80.2
petG-trnW-CCA_p2	GCGATCAGTTGGACCTTTGA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.2	58.0	221-222 bp	1.0	80.1
petG-trnW-CCA_p3	GCGATCAGTTGGACCTTTGA	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.2	57.9	185-186 bp	1.0	79.9
petG-trnW-CCA_p4	GCGATCAGTTGGACCTTTGA	CCCGATGTCGTAGGTTCAAA	50.0	50.0	58.2	57.6	180-181 bp	1.0	77.7
petG-trnW-CCA_p5	GCGATCAGTTGGACCTTTGA	CGCTCTTAGTTCAGTTCGGT	50.0	50.0	58.2	57.7	219-220 bp	1.0	77.5