Hotspot

ycf4-cemA

LSC · 60635-61294 · 660 bp

Back to Collinsonia

Pi 0.00808

MarkerSeek score 61.80

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 660
variable_sites: 8
pi: 0.00808
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGCGCGTACCAATTGAAGTA	GAAATCCACCAGGGCAGAAA	45.0	50.0	57.9	58.1	755-757 bp	1.0	79.8
ycf4-cemA_p2	CTTGCGCGTACCAATTGAAG	GAAATCCACCAGGGCAGAAA	50.0	50.0	58.1	58.1	757-759 bp	1.0	79.5
ycf4-cemA_p3	GAACAAAAAGCCGCGGAATT	GAAATCCACCAGGGCAGAAA	45.0	50.0	58.2	58.1	786-788 bp	1.0	78.6
ycf4-cemA_p4	TTTCTTGCGCGTACCAATTG	GAAATCCACCAGGGCAGAAA	45.0	50.0	57.7	58.1	760-762 bp	1.0	77.8
ycf4-cemA_p5	CCTATTTCTTGCGCGTACCA	GAAATCCACCAGGGCAGAAA	50.0	50.0	58.4	58.1	764-766 bp	1.0	77.6