Hotspot

ndhC-trnV-UAC

LSC · 50365-51357 · 993 bp

Back to Collinsonia

Pi 0.0105

MarkerSeek score 62.30

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 993
variable_sites: 15
pi: 0.0105
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CGGGGCTAAAATCCCAGAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1105-1126 bp	1.0	79.8
ndhC-trnV-UAC_p2	AATCGGGGCTAAAATCCCAG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.6	57.8	1108-1129 bp	1.0	78.0
ndhC-trnV-UAC_p3	CTCCGGCCCTTTTCTAATCG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.4	57.8	1123-1144 bp	1.0	77.5
ndhC-trnV-UAC_p4	CGGGGCTAAAATCCCAGAAA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	1104-1125 bp	1.0	77.1
ndhC-trnV-UAC_p5	CGGGGCTAAAATCCCAGAAA	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	1093-1114 bp	1.0	76.0