Hotspot

rps4-trnT-UGU

LSC · 45839-46291 · 453 bp

Back to Collinsonia

Pi 0.0191

MarkerSeek score 67.20

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 453
variable_sites: 13
pi: 0.0191
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TCTAAGATCACTCCCGGCTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.8	57.9	613-623 bp	1.0	81.3
rps4-trnT-UGU_p2	TTCTAAGATCACTCCCGGCT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.8	57.9	614-624 bp	1.0	81.3
rps4-trnT-UGU_p3	TCTAAGATCACTCCCGGCTT	ATGGTCATCGGTTCGATTCC	50.0	50.0	57.8	57.8	581-591 bp	1.0	80.6
rps4-trnT-UGU_p4	TTCTAAGATCACTCCCGGCT	ATGGTCATCGGTTCGATTCC	50.0	50.0	57.8	57.8	582-592 bp	1.0	80.6
rps4-trnT-UGU_p5	GTTAGTCCTGGTAAAGCCCC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	57.6	57.9	579-589 bp	1.0	79.5