Hotspot

trnL-UAG

SSC · 116945-117024 · 80 bp

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Pi 0.592

MarkerSeek score 77.30

Primer available yes

Label trnL-UAG

Feature Metadata

label_name: trnL-UAG
feature_type: tRNA
region: SSC
length_bp: 80
variable_sites: 45
pi: 0.592
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG_p1	GTGTTGGGTCTCAAATGGGT	ATCCGCATAAAAGAGCTGCA	50.0	45.0	58.0	58.0	975 bp	0.5	59.6
trnL-UAG_p2	GTGTTGGGTCTCAAATGGGT	TCCGCATAAAAGAGCTGCAT	50.0	45.0	58.0	58.0	974 bp	0.5	59.6
trnL-UAG_p3	GTGTTGGGTCTCAAATGGGT	TGGCGAAACCTTGGGTAAAA	50.0	45.0	58.0	57.9	855 bp	0.5	59.4
trnL-UAG_p4	ATACCCCGACTGAGAACGAA	ATCCGCATAAAAGAGCTGCA	50.0	45.0	58.2	58.0	924 bp	0.5	58.4
trnL-UAG_p5	ATACCCCGACTGAGAACGAA	TCCGCATAAAAGAGCTGCAT	50.0	45.0	58.2	58.0	923 bp	0.5	58.4