Hotspot

ndhH

SSC · 124933-126114 · 1182 bp

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Pi 0.466

MarkerSeek score 80.60

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 459
pi: 0.466
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	CCAGCATATTCAGGCCCAAT	TTCTGGGAAAACGTCAACGA	50.0	45.0	58.0	57.7	1656 bp	0.5	57.5
ndhH_p2	TTGGTACCATCCGCTAAAGC	TTCTGGGAAAACGTCAACGA	50.0	45.0	58.0	57.7	1695 bp	0.5	57.2
ndhH_p3	TGTTCCAACGAATCACACGT	TTCTGGGAAAACGTCAACGA	45.0	45.0	58.1	57.7	2024 bp	0.5	57.0
ndhH_p4	AAAGGGCCAGCATATTCAGG	TTCTGGGAAAACGTCAACGA	50.0	45.0	57.9	57.7	1662 bp	0.5	56.9
ndhH_p5	CCAGCATATTCAGGCCCAAT	TCTGGGAAAACGTCAACGAT	50.0	45.0	58.0	57.5	1655 bp	0.5	55.7