Hotspot

nad6

SSC · 121319-121849 · 531 bp

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Pi 0.471

MarkerSeek score 78.80

Primer available yes

Label nad6

Feature Metadata

label_name: nad6
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 208
pi: 0.471
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
nad6_p1	TCGGAAAATGCGACGAGATT	TTTTGCGGTAACTGTGTCGA	45.0	45.0	58.0	58.1	1273 bp	0.5	59.5
nad6_p2	AGTGCTCTAACCATGCTTCG	TTTTGCGGTAACTGTGTCGA	50.0	45.0	58.0	58.1	1216 bp	0.5	59.4
nad6_p3	TCGGAAAATGCGACGAGATT	ATGTGTTCGCGTATGTCCAA	45.0	45.0	58.0	57.9	1382 bp	0.5	59.3
nad6_p4	TCGGAAAATGCGACGAGATT	TGTGTTCGCGTATGTCCAAT	45.0	45.0	58.0	57.9	1381 bp	0.5	59.3
nad6_p5	CTCTAACCATGCTTCGGCTT	TTTTGCGGTAACTGTGTCGA	50.0	45.0	58.0	58.1	1212 bp	0.5	59.3