Hotspot

ccsA

SSC · 117156-118124 · 969 bp

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Pi 0.457

MarkerSeek score 79.30

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 353
pi: 0.457
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGTTGGGTCTCAAATGGGT	TCTGGACCACGAGAACTCTT	50.0	50.0	58.0	58.0	1888 bp	0.5	59.9
ccsA_p2	GTGTTGGGTCTCAAATGGGT	TTCTGGACCACGAGAACTCT	50.0	50.0	58.0	58.0	1889 bp	0.5	59.9
ccsA_p3	GTGTTGGGTCTCAAATGGGT	TTCTCGATGGCTTCTCTTGC	50.0	50.0	58.0	58.0	2116 bp	0.5	59.8
ccsA_p4	ATTTAGGGTAAGCCGCCATG	TCTGGACCACGAGAACTCTT	50.0	50.0	58.0	58.0	1595 bp	0.5	59.8
ccsA_p5	ATTTAGGGTAAGCCGCCATG	TTCTGGACCACGAGAACTCT	50.0	50.0	58.0	58.0	1596 bp	0.5	59.8