Hotspot

ndhI-ndhA

SSC · 122580-122699 · 120 bp

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Pi 0.393

MarkerSeek score 75.00

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 120
variable_sites: 46
pi: 0.393
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	TCGACACAGTTACCGCAAAA	TTTGGAATAGCGATGGGAGC	45.0	50.0	58.1	58.0	1038 bp	0.5	59.2
ndhI-ndhA_p2	TCGACACAGTTACCGCAAAA	ACCAGAAGCAGAGGAGGAAT	45.0	50.0	58.1	58.0	849 bp	0.5	59.2
ndhI-ndhA_p3	ATTGGACATACGCGAACACA	TTTGGAATAGCGATGGGAGC	45.0	50.0	57.9	58.0	930 bp	0.5	59.0
ndhI-ndhA_p4	TTGGACATACGCGAACACAT	TTTGGAATAGCGATGGGAGC	45.0	50.0	57.9	58.0	929 bp	0.5	59.0
ndhI-ndhA_p5	TTGGACATACGCGAACACAT	ACCAGAAGCAGAGGAGGAAT	45.0	50.0	57.9	58.0	740 bp	0.5	59.0