Hotspot

nad6-ndhI

SSC · 121850-122075 · 226 bp

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Pi 0.481

MarkerSeek score 77.30

Primer available yes

Label nad6-ndhI

Feature Metadata

label_name: nad6-ndhI
feature_type: igs
region: SSC
length_bp: 226
variable_sites: 103
pi: 0.481
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
nad6-ndhI_p1	GCAGAATCGATGGGATTGGT	TTTTGCGGTAACTGTGTCGA	50.0	45.0	58.0	58.1	522 bp	0.5	59.3
nad6-ndhI_p2	GCAGAATCGATGGGATTGGT	ATGTGTTCGCGTATGTCCAA	50.0	45.0	58.0	57.9	631 bp	0.5	59.1
nad6-ndhI_p3	GCAGAATCGATGGGATTGGT	TGTGTTCGCGTATGTCCAAT	50.0	45.0	58.0	57.9	630 bp	0.5	59.1
nad6-ndhI_p4	GCAGAATCGATGGGATTGGT	CAAACAATACGAGCCGCAAG	50.0	50.0	58.0	58.1	798 bp	0.5	58.8
nad6-ndhI_p5	GCAGAATCGATGGGATTGGT	TGTGAAGTATGTGTTCGCGT	50.0	45.0	58.0	57.9	639 bp	0.5	58.6