Hotspot

psaC-ndhE

SSC · 120275-120834 · 560 bp

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Pi 0.393

MarkerSeek score 79.30

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 560
variable_sites: 182
pi: 0.393
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GGACAAGCGGATTCACATCT	AATCTCGTCGCATTTTCCGA	50.0	45.0	58.0	58.0	913 bp	0.5	59.7
psaC-ndhE_p2	GGACAAGCGGATTCACATCT	CGAAGCATGGTTAGAGCACT	50.0	50.0	58.0	58.0	970 bp	0.5	59.6
psaC-ndhE_p3	TGAAGAACCCGCGAATATGG	AATCTCGTCGCATTTTCCGA	50.0	45.0	58.1	58.0	1175 bp	0.5	59.5
psaC-ndhE_p4	GGACAAGCGGATTCACATCT	AAGCCGAAGCATGGTTAGAG	50.0	50.0	58.0	58.0	974 bp	0.5	59.5
psaC-ndhE_p5	TGAAGAACCCGCGAATATGG	CGAAGCATGGTTAGAGCACT	50.0	50.0	58.1	58.0	1232 bp	0.5	59.5