Hotspot

rpl32-trnL-UAG

SSC · 116252-116944 · 693 bp

Back to Collabium

Pi 0.472

MarkerSeek score 82.70

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 693
variable_sites: 300
pi: 0.472
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TCCACCGGACAGTCAAAAAG	ATCCGCATAAAAGAGCTGCA	50.0	45.0	58.0	58.0	1424 bp	0.5	59.4
rpl32-trnL-UAG_p2	TCCACCGGACAGTCAAAAAG	TCCGCATAAAAGAGCTGCAT	50.0	45.0	58.0	58.0	1423 bp	0.5	59.4
rpl32-trnL-UAG_p3	TCCACCGGACAGTCAAAAAG	TGGCGAAACCTTGGGTAAAA	50.0	45.0	58.0	57.9	1304 bp	0.5	59.1
rpl32-trnL-UAG_p4	TCCACCGGACAGTCAAAAAG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	792 bp	0.5	58.7
rpl32-trnL-UAG_p5	TCCACCGGACAGTCAAAAAG	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.0	57.8	785 bp	0.5	58.2