Hotspot

ndhF-rpl32

SSC · 115051-116077 · 1027 bp

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Pi 0.421

MarkerSeek score 79.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1027
variable_sites: 352
pi: 0.421
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACAGATTGGCCGAAAACCAT	ACCCATTTGAGACCCAACAC	45.0	50.0	58.1	58.0	1785 bp	0.5	59.3
ndhF-rpl32_p2	AGCAACTGCACCGACAAATA	ACCCATTTGAGACCCAACAC	45.0	50.0	58.1	58.0	2327 bp	0.5	59.1
ndhF-rpl32_p3	ATTTAGCAACTGCACCGACA	ACCCATTTGAGACCCAACAC	45.0	50.0	58.1	58.0	2331 bp	0.5	59.1
ndhF-rpl32_p4	TAGCAACTGCACCGACAAAT	ACCCATTTGAGACCCAACAC	45.0	50.0	58.1	58.0	2328 bp	0.5	59.1
ndhF-rpl32_p5	ACAGATTGGCCGAAAACCAT	CTTTTTGACTGTCCGGTGGA	45.0	50.0	58.1	58.0	1336 bp	0.5	59.1