Hotspot

psbC-trnS-UGA

LSC · 36407-36551 · 145 bp

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Pi 0.0483

MarkerSeek score 80.30

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 145
variable_sites: 7
pi: 0.0483
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.2	58.0	267 bp	1.0	80.6
psbC-trnS-UGA_p2	CCTGTTCTTTCCATGACCCC	ATAGCCCCGGTCTTGAAAAC	55.0	50.0	58.2	57.9	247 bp	1.0	80.0
psbC-trnS-UGA_p3	AGCGGGGTTTGAAAAAGGAA	GAGAGATGGCTGAGTGGTTG	45.0	55.0	58.2	58.0	304 bp	1.0	79.9
psbC-trnS-UGA_p4	TTCCATGACCCCTCTTAGCT	GAGAGATGGCTGAGTGGTTG	50.0	55.0	57.7	58.0	259 bp	1.0	79.8
psbC-trnS-UGA_p5	TTGTGGGACATTTGTGGCAT	GAGAGATGGCTGAGTGGTTG	45.0	55.0	58.3	58.0	347 bp	1.0	79.2