Hotspot

ycf1

SSC · 122322-127898 · 5577 bp

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Pi 0.0188
MarkerSeek score 51.60
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5577
variable_sites
202
pi
0.0188
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 GGAGATCCCGATTCTCTAACA AAACCTTTCACCTTCAACCT 47.6 40.0 56.3 55.0 210 bp 1.0 46.5
ycf1_p2 GGAGATCCCGATTCTCTAACA AACCTTTCACCTTCAACCTT 47.6 40.0 56.3 55.0 209 bp 1.0 46.5
ycf1_p3 GGAGATCCCGATTCTCTAACA AAACCTTTCACCTTCAACCTT 47.6 38.1 56.3 55.8 210 bp 1.0 46.5
ycf1_p4 GGAGATCCCGATTCTCTAACA CAAAAACCTTTCACCTTCAACCT 47.6 39.1 56.3 57.9 213 bp 1.0 46.4
ycf1_p5 GGAGATCCCGATTCTCTAACA ACAAAAACCTTTCACCTTCAACC 47.6 39.1 56.3 58.2 214 bp 1.0 46.4