Hotspot

rpl16

LSC · 80570-81835 · 1266 bp

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Pi 0.0194
MarkerSeek score 58.10
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1266
variable_sites
47
pi
0.0194
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 CATATCGGCGATTGCTAGCT CGACGGAAAAGAAATTGCCC 50.0 50.0 58.0 58.0 1790-1799 bp 1.0 83.1
rpl16_p2 CATATCGGCGATTGCTAGCT GACGGAAAAGAAATTGCCCG 50.0 50.0 58.0 58.0 1789-1798 bp 1.0 83.1
rpl16_p3 GAGCATATCGGCGATTGCTA GACGGAAAAGAAATTGCCCG 50.0 50.0 58.0 58.0 1792-1801 bp 0.75 71.3
rpl16_p4 AGCATATCGGCGATTGCTAG CGACGGAAAAGAAATTGCCC 50.0 50.0 58.0 58.0 1792-1801 bp 0.75 71.2
rpl16_p5 AGCATATCGGCGATTGCTAG GACGGAAAAGAAATTGCCCG 50.0 50.0 58.0 58.0 1791-1800 bp 0.75 71.2