Hotspot

ndhF

SSC · 109075-111306 · 2232 bp

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Pi 0.0163
MarkerSeek score 55.40
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2232
variable_sites
69
pi
0.0163
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 GGCGGATCCAAACAAAATCG CGCCCAATACCCTTTCCTTT 50.0 50.0 58.1 58.2 2928-2948 bp 1.0 81.2
ndhF_p2 GGCGGATCCAAACAAAATCG CTTTTAACGCCGCCCAATAC 50.0 50.0 58.1 57.8 2938-2958 bp 1.0 80.9
ndhF_p3 GGAAGAAAAGGCGGATCCAA CGCCCAATACCCTTTCCTTT 50.0 50.0 58.2 58.2 2937-2957 bp 1.0 80.6
ndhF_p4 GGAAGAAAAGGCGGATCCAA CTTTTAACGCCGCCCAATAC 50.0 50.0 58.2 57.8 2947-2967 bp 1.0 80.3
ndhF_p5 GACGATCCTCCTCCTTCCTT CGCCCAATACCCTTTCCTTT 55.0 50.0 58.2 58.2 2963-2983 bp 1.0 80.1