Hotspot

ndhD

SSC · 114076-115578 · 1503 bp

Pi 0.0118

MarkerSeek score 52.70

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 33
pi: 0.0118
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CTAATGAAGCATGGGGGTCC	TTTTTCTGGCCCAAGCGTAT	55.0	45.0	58.0	58.1	1987-2000 bp	1.0	82.2
ndhD_p2	GAAGCATGGGGGTCCTATTG	TTTTTCTGGCCCAAGCGTAT	55.0	45.0	58.0	58.1	1982-1995 bp	1.0	82.2
ndhD_p3	CTAATGAAGCATGGGGGTCC	CTGCGTTGGTTGTAAGAGGT	55.0	50.0	58.0	58.1	2178-2191 bp	1.0	82.0
ndhD_p4	GAAGCATGGGGGTCCTATTG	ACTGCGTTGGTTGTAAGAGG	55.0	50.0	58.0	58.1	2174-2187 bp	1.0	82.0
ndhD_p5	GAAGCATGGGGGTCCTATTG	CTGCGTTGGTTGTAAGAGGT	55.0	50.0	58.0	58.1	2173-2186 bp	1.0	82.0