Hotspot

ccsA-ndhD

SSC · 113853-114075 · 223 bp

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Pi 0.08
MarkerSeek score 75.20
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
223
variable_sites
31
pi
0.08
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 GAAGCATGGGGGTCCTATTG CAGTAGCTTTTCGATGGCCT 55.0 50.0 58.0 58.0 817-830 bp 1.0 84.2
ccsA-ndhD_p2 CTAATGAAGCATGGGGGTCC TCAGTAGCTTTTCGATGGCC 55.0 50.0 58.0 58.0 824-836 bp 0.75 72.2
ccsA-ndhD_p3 GAAGCATGGGGGTCCTATTG TCAGTAGCTTTTCGATGGCC 55.0 50.0 58.0 58.0 819-831 bp 0.75 72.2
ccsA-ndhD_p4 CTAATGAAGCATGGGGGTCC AACGGGGCCCTTTTACAAAT 55.0 45.0 58.0 58.0 972-984 bp 0.75 72.0
ccsA-ndhD_p5 GAAGCATGGGGGTCCTATTG AACGGGGCCCTTTTACAAAT 55.0 45.0 58.0 58.0 967-979 bp 0.75 72.0