Hotspot

rpl32-trnL-UAG

SSC · 111952-112716 · 765 bp

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Pi 0.0286
MarkerSeek score 71.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
765
variable_sites
41
pi
0.0286
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TTTTTGTGCGACAAACAAAT TCCTAAGAGCAGCGTGTCTA 30.0 50.0 53.4 57.9 749-836 bp 0.75 39.8
rpl32-trnL-UAG_p2 TTTTTGTGCGACAAACAAAT AGCAGCGTGTCTACCAATTT 30.0 45.0 53.4 57.8 742-829 bp 0.75 39.4
rpl32-trnL-UAG_p3 TTTTTGTGCGACAAACAAAT CCTAAGAGCAGCGTGTCTAC 30.0 55.0 53.4 57.8 748-835 bp 0.75 39.3
rpl32-trnL-UAG_p4 TTTTTGTGCGACAAACAAAT CTAAGAGCAGCGTGTCTACC 30.0 55.0 53.4 57.8 747-834 bp 0.75 39.3
rpl32-trnL-UAG_p5 TTTTTGTGCGACAAACAAAT GAGCAGCGTGTCTACCAATT 30.0 50.0 53.4 58.3 743-830 bp 0.75 38.7