Hotspot

rpl22-rps19

LSC · 83100-83168 · 69 bp

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Pi 0.07
MarkerSeek score 69.10
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
LSC
length_bp
69
variable_sites
9
pi
0.07
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 CGCATAACTACGTCCTCGAG TCGTCGCCGTAGTAAGTAGT 55.0 50.0 58.0 58.0 734-741 bp 1.0 83.1
rpl22-rps19_p2 GAACGTCCGCGAATTTGATC TCGTCGCCGTAGTAAGTAGT 50.0 50.0 57.9 58.0 532-539 bp 1.0 83.1
rpl22-rps19_p3 CGCATAACTACGTCCTCGAG TTGTTCGTCGCCGTAGTAAG 55.0 50.0 58.0 58.0 738-745 bp 1.0 82.9
rpl22-rps19_p4 TGTCGGCCTAAAGCACATAC TCGTCGCCGTAGTAAGTAGT 50.0 50.0 58.0 58.0 481-482 bp 0.75 70.3
rpl22-rps19_p5 TGTCGGCCTAAAGCACATAC TTGTTCGTCGCCGTAGTAAG 50.0 50.0 58.0 58.0 485-486 bp 0.75 70.1