Hotspot

psbB-psbT

LSC · 73475-73647 · 173 bp

Back to Coleus

Pi 0.0405
MarkerSeek score 70.10
Primer available yes
Label psbB-psbT

Feature Metadata

label_name
psbB-psbT
feature_type
igs
region
LSC
length_bp
173
variable_sites
12
pi
0.0405
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbB-psbT_p1 GTACGTCGTATGCCCACTTT GCAAATAGAGCCATTGCGAC 50.0 50.0 58.0 57.9 1188-1202 bp 1.0 81.3
psbB-psbT_p2 TTCTTCGGACACATTTGGCA GAAGTAATGAGCCTCCCAGC 45.0 55.0 58.0 58.0 479-480 bp 0.5 60.3
psbB-psbT_p3 GTACGTCGTATGCCCACTTT GAAGTAATGAGCCTCCCAGC 50.0 55.0 58.0 58.0 800-801 bp 0.5 60.2
psbB-psbT_p4 ACGGAGATGGAATAGCGGTA GAAGTAATGAGCCTCCCAGC 50.0 55.0 58.0 58.0 871-872 bp 0.5 60.2
psbB-psbT_p5 ATGCTTCGTTTGCTTTGCTC GAAGTAATGAGCCTCCCAGC 45.0 55.0 57.9 58.0 502-503 bp 0.5 60.0