Hotspot

pafI-trnS-GGA

LSC · 44011-44872 · 862 bp

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Pi 0.0169
MarkerSeek score 67.60
Primer available yes
Label pafI-trnS-GGA

Feature Metadata

label_name
pafI-trnS-GGA
feature_type
igs
region
LSC
length_bp
862
variable_sites
28
pi
0.0169
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
pafI-trnS-GGA_p1 TGCCTCCTTTTCTCCTGAAG CCCTCGGTAAACAAAAGCCT 50.0 50.0 57.1 58.1 1032-1042 bp 1.0 75.8
pafI-trnS-GGA_p2 GCCTCCTTTTCTCCTGAAGT CCCTCGGTAAACAAAAGCCT 50.0 50.0 57.1 58.1 1031-1041 bp 1.0 75.8
pafI-trnS-GGA_p3 TGCCTCCTTTTCTCCTGAAG TGAAAGTGCGGAAAGAGAGG 50.0 50.0 57.1 57.8 1060-1070 bp 1.0 75.1
pafI-trnS-GGA_p4 GCCTCCTTTTCTCCTGAAGT TGAAAGTGCGGAAAGAGAGG 50.0 50.0 57.1 57.8 1059-1069 bp 1.0 75.1
pafI-trnS-GGA_p5 TGCCTCCTTTTCTCCTGAAG TTCCAATGCTACGCCTTGAA 50.0 45.0 57.1 57.8 1003-1013 bp 1.0 75.1