Hotspot

rps2-rpoC2

LSC · 16226-16434 · 209 bp

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Pi 0.0383
MarkerSeek score 68.00
Primer available yes
Label rps2-rpoC2

Feature Metadata

label_name
rps2-rpoC2
feature_type
igs
region
LSC
length_bp
209
variable_sites
15
pi
0.0383
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2-rpoC2_p1 CCTCCAGCATCTCTTCCAAG CAATCATTTATAGGATTTAATGATTCC 55.0 25.9 57.7 53.5 282-293 bp 1.0 45.0
rps2-rpoC2_p2 CCTCCAGCATCTCTTCCAAG CAGAACAATCATTTATAGGATTTAATG 55.0 25.9 57.7 53.7 287-298 bp 1.0 44.9
rps2-rpoC2_p3 AAATGAACTCCTGCCTCCAG CAATCATTTATAGGATTTAATGATTCC 50.0 25.9 57.5 53.5 295-306 bp 1.0 44.8
rps2-rpoC2_p4 AAATGAACTCCTGCCTCCAG CAGAACAATCATTTATAGGATTTAATG 50.0 25.9 57.5 53.7 300-311 bp 1.0 44.7
rps2-rpoC2_p5 CCATGGCCAAAATGAACTCC CAGAACAATCATTTATAGGATTTAATG 50.0 25.9 57.3 53.7 309-320 bp 1.0 44.6