Hotspot

trnK-rps16

LSC · 4251-4778 · 528 bp

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Pi 0.0316
MarkerSeek score 65.80
Primer available yes
Label trnK-rps16

Feature Metadata

label_name
trnK-rps16
feature_type
igs
region
LSC
length_bp
528
variable_sites
33
pi
0.0316
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-rps16_p1 ATTCACTCAAGTGCTTCCCG AAAAGGAGCTCAACCCACAG 50.0 50.0 58.2 58.0 1022-1030 bp 1.0 82.2
trnK-rps16_p2 ATTCACTCAAGTGCTTCCCG CCGGGATAAACGAAAGGGAG 50.0 55.0 58.2 58.1 1468-1477 bp 1.0 81.4
trnK-rps16_p3 ATTCACTCAAGTGCTTCCCG GCCGTACGAGGAGAAAACTT 50.0 50.0 58.2 57.9 1222-1231 bp 1.0 81.2
trnK-rps16_p4 ATTCACTCAAGTGCTTCCCG TCTATCCCAATGAGCCGTCT 50.0 50.0 58.2 57.9 1166-1174 bp 1.0 81.2
trnK-rps16_p5 AGGTAGAAGATTCGGGGGTT AAAAGGAGCTCAACCCACAG 50.0 50.0 57.7 58.0 1341-1353 bp 1.0 80.7