Hotspot

trnH-GUG-psbA

LSC · 86-379 · 294 bp

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Pi 0.042
MarkerSeek score 70.90
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
294
variable_sites
24
pi
0.042
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TCAGTGCTATGCATGGTTCC 55.0 50.0 58.1 58.0 744-760 bp 1.0 83.2
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG CAGTGCTATGCATGGTTCCT 55.0 50.0 58.1 58.0 743-759 bp 1.0 83.2
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA TCAGTGCTATGCATGGTTCC 50.0 50.0 57.9 58.0 758-774 bp 1.0 83.1
trnH-GUG-psbA_p4 AATCCACTGCCTTGATCCAC TCAGTGCTATGCATGGTTCC 50.0 50.0 57.9 58.0 757-773 bp 1.0 83.1
trnH-GUG-psbA_p5 ACAATCCACTGCCTTGATCC TCAGTGCTATGCATGGTTCC 50.0 50.0 57.9 58.0 759-775 bp 1.0 83.1