Hotspot

ftsH

Genome · 90138-95240 · 5103 bp

Back to Coleochaete

Pi 0.0268

MarkerSeek score 54.40

Primer available yes

Label ftsH

Feature Metadata

label_name: ftsH
feature_type: gene
region: Genome
length_bp: 5103
variable_sites: 137
pi: 0.0268
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ftsH_p1	ACACAAAAAGATTCGGTTGCT	AGCGAATTCCACCATTCCAA	38.1	45.0	56.9	57.8	1331 bp	0.5	41.7
ftsH_p2	ACACAAAAAGATTCGGTTGC	AGCGAATTCCACCATTCCAA	40.0	45.0	55.4	57.8	1331 bp	0.5	37.4
ftsH_p3	CACAAAAAGATTCGGTTGCT	AGCGAATTCCACCATTCCAA	40.0	45.0	55.1	57.8	1330 bp	0.5	35.0
ftsH_p4	ACACAAAAAGATTCGGTTGCT	TAGCGAATTCCACCATTCCA	38.1	45.0	56.9	56.9	1332 bp	0.5	34.7
ftsH_p5	TTCGGTTGCTATTCCAATCA	AGCGAATTCCACCATTCCAA	40.0	45.0	55.0	57.8	1320 bp	0.5	34.6