Hotspot

psbJ-petA

Genome · 102569-103284 · 716 bp

Back to Coleochaete

Pi 0.0251

MarkerSeek score 73.20

Primer available yes

Label psbJ-petA

Feature Metadata

label_name: psbJ-petA
feature_type: igs
region: Genome
length_bp: 716
variable_sites: 18
pi: 0.0251
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-petA_p1	AGGCAGAATTCCTCTTTGGC	TCCTGCTAGATTGCAAGGTT	50.0	45.0	58.2	56.8	943-944 bp	1.0	70.8
psbJ-petA_p2	GGCAGAATTCCTCTTTGGCT	TCCTGCTAGATTGCAAGGTT	50.0	45.0	58.2	56.8	942-943 bp	1.0	70.8
psbJ-petA_p3	AGGCAGAATTCCTCTTTGGC	GATCCTGCTAGATTGCAAGGT	50.0	47.6	58.2	57.8	945-946 bp	1.0	70.6
psbJ-petA_p4	GGCAGAATTCCTCTTTGGCT	GATCCTGCTAGATTGCAAGGT	50.0	47.6	58.2	57.8	944-945 bp	1.0	70.6
psbJ-petA_p5	GGCAGAATTCCTCTTTGGCT	ATCCTGCTAGATTGCAAGGT	50.0	45.0	58.2	56.6	943-944 bp	1.0	69.0