Hotspot

tRNA-Ser-psbZ

Genome · 99328-99655 · 328 bp

Back to Coleochaete

Pi 0.0382

MarkerSeek score 70.50

Primer available yes

Label tRNA-Ser-psbZ

Feature Metadata

label_name: tRNA-Ser-psbZ
feature_type: igs
region: Genome
length_bp: 328
variable_sites: 12
pi: 0.0382
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Ser-psbZ_p1	GCCATAAACCACTCGACCAT	TCCATCTGGAGAAGCAAGTAC	50.0	47.6	58.0	57.1	436-450 bp	1.0	69.2
tRNA-Ser-psbZ_p2	GCCATAAACCACTCGACCAT	CCATCTGGAGAAGCAAGTACT	50.0	47.6	58.0	57.1	435-449 bp	1.0	69.2
tRNA-Ser-psbZ_p3	ATGGGAGAGAGAGGGATTCG	TCCATCTGGAGAAGCAAGTAC	55.0	47.6	58.0	57.1	496-510 bp	1.0	68.9
tRNA-Ser-psbZ_p4	ATGGGAGAGAGAGGGATTCG	CCATCTGGAGAAGCAAGTACT	55.0	47.6	58.0	57.1	495-509 bp	1.0	68.9
tRNA-Ser-psbZ_p5	GGGAGAGAGAGGGATTCGAA	TCCATCTGGAGAAGCAAGTAC	55.0	47.6	57.9	57.1	494-508 bp	1.0	68.4