Hotspot

tRNA-Leu-tRNA-Asn

Genome · 67000-67086 · 87 bp

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Pi 0.0805
MarkerSeek score 75.10
Primer available yes
Label tRNA-Leu-tRNA-Asn

Feature Metadata

label_name
tRNA-Leu-tRNA-Asn
feature_type
igs
region
Genome
length_bp
87
variable_sites
7
pi
0.0805
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Leu-tRNA-Asn_p1 GCTTAAGCATCTCGGTTCGA GGTTCAAATCCTACCTGGGG 50.0 55.0 58.1 57.6 144 bp 1.0 79.8
tRNA-Leu-tRNA-Asn_p2 TAGACACGCTGCTCTTAGGA GGTTCAAATCCTACCTGGGG 50.0 55.0 57.9 57.6 170 bp 1.0 78.8
tRNA-Leu-tRNA-Asn_p3 GCTTAAGCATCTCGGTTCGA GGCTGTTAACCGATTTGTCG 50.0 50.0 58.1 57.5 166 bp 1.0 78.6
tRNA-Leu-tRNA-Asn_p4 GGTAGACACGCTGCTCTTAG GGTTCAAATCCTACCTGGGG 55.0 55.0 57.8 57.6 172 bp 1.0 78.2
tRNA-Leu-tRNA-Asn_p5 GTAGACACGCTGCTCTTAGG GGTTCAAATCCTACCTGGGG 55.0 55.0 57.8 57.6 171 bp 1.0 78.2